Past Solicitations
BAA 14-003 Call 0014
Bioforensics Research and Development (R&D) Whole Genome Approach

Research and development of next generation and novel technologies to characterize biological and chemical threat agents for source attribution in support of FBI and NBFAC requirements in a criminal investigation. These include novel technologies to characterize the organism, the agent, or the sample matrix.

Topics

TTA-1 - Identify and sequence near neighbors of Francisella tularensis

DHS S&T seeks proposals to identify and sequence near neighbors of Francisella tularensis. The goal of TTA-1 is to develop data that will support an increased specificity of molecular assays for the identification of pathogenic Francisella tularensis strains at the exclusion of near neighbors. The plan should identify those isolates that are available and include a plan to sequence them, as well as strategies to isolate and sequence near neighbors from environmental isolates.

TTA-2 - Metagenomics analysis of complex biological samples

DHS S&T seeks proposals to develop methods that will be transitioned to operational laboratories for sample prep methods that will support metagenomic analysis of complex biological samples. TTA-2 will be focused on the development of methods for sample preparation of complex samples containing mixtures of eukaryotic (plant, animal and fungal), prokaryotic, and viral DNA and RNA to support whole genome sequencing. It is the goal of DHS that the methods developed will provide for a non-biased isolation DNA and RNA to support downstream analysis of whole genome sequencing platforms to include, but not limited to, Miseq, Hiseq and Pac Bio. The end product should provide DHS with protocols that include quantitative limits for the isolation of eukaryotic (plant, animal and fungal), prokaryotic, and viral DNA and RNA in complex backgrounds.

TTA-3 - High confidence metagenomics analysis of complex samples

DHS S&T seeks proposals to provide a foundation for high confidence metagenomic analysis of complex samples that will be transitioned to operational laboratories. TTA-3 is focused on the development of informatics and statistical tools needed to identify and characterize complex biological mixtures of eukaryotic (plant, animal and fungal), prokaryotic, and viral DNA and RNA, excluding clinical samples. The input data would be from genome sequencing platforms that include but are not limited to Miseq, Hiseq, and Pac Bio. There is no limitation on computational support needed to achieve this task. It is anticipated that the final product will provide DHS with the tools needed to identify the constituents (based on the DNA and RNA) of complex biological samples and provide robust confidence levels for the identification each biological agent. DHS will not entertain proposals that are based on 16 S ribosomal DNA methods.

Key Dates
Solicitation Open Date:
12/09/2015
White Paper Registration Deadline:
01/07/2016 03:00 PM ET
White Paper Submission Deadline:
01/07/2016 03:00 PM ET
Submission Deadline:
03/08/2016 12:00 PM ET


Amendments and Q&As
amendment icon Amendment # 2
Posted Date 12/18/15

( The purpose of this Amendment 2 is to Post Questions and Answers with updated Call 14 Announcement, ...)
(Read Full)

The purpose of this Amendment 2 is to Post Questions and Answers with updated Call 14 Announcement, Dated December 18, 2015.


amendment icon Amendment # 1
Posted Date 12/11/15
The purpose of this Amendment 1 is to remove and replace the draft water marked document.



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