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The performer will test, optimize, evaluate, and characterize prototype assays for further validation against the panel of Government determined inclusivity and exclusivity materials. The performer will work with Government guidance to validate the final assays, and shall have the capability of commercially and rapidly producing them for sale to other designated end users. DHS may also conduct internal, follow-on specialized and specific analysis, test, evaluation, and validation of assays to help characterize and deploy the assays for use by DHS customers with areas of responsibility for biological defense, detection, surveillance, and response. Prototype assays for this purpose will be included in the final deliverables of the assays.