The mission of the Department of Homeland Security (DHS), S&T/CBD is to provide technologies, methods, and solutions to enable the detection of biological agents of concern in a timely manner, and to identify the target agents with a high degree of confidence. As the threat of bioterrorism evolves, DHS intends to assess a wide spectrum of potential technologies that can enhance its capabilities. DHS's mission space includes preventing, detecting, responding to, and recovering from intentional or accidental introduction of biological and chemical agents which present a threat against the Nation's human population and critical infrastructure. To support this mission, DHS and its state and local partners have a need to quickly collect reliable information to enable a swift and confident response to a biological and/or chemical threat. The Chemical and Biological Defense (CBD) Division within DHS S&T is working toward developing detection assays for the identification of Bacterial Select Agents. Areas-of-responsibility associated with this BAA Call include the development, testing and validation, and long-term maintenance of the assays suite to ensure their reliability and accuracy.